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goat polyclonal anti chi3l1  (R&D Systems)


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    R&D Systems goat polyclonal anti chi3l1
    Goat Polyclonal Anti Chi3l1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+a+human+chi3l1/Human%2FPrimate+Chitinase+3-like+1%2FYKL-40+Biotinylated+Antibody/pmc12985343-173-10-16
    Average 94 stars, based on 1 article reviews
    goat polyclonal anti chi3l1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.
    Article Snippet: After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.. The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.. The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.

    Incubation:

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.
    Article Snippet: After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.. The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.. The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.

    Quantitative RT-PCR:

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.
    Article Snippet: After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.. The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.. The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.

    Western Blot:

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.
    Article Snippet: After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.. The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.. The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.

    Immunocytochemistry:

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.
    Article Snippet: After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.. The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.. The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.

    In Vitro:

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.
    Article Snippet: After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.. The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.. The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.

    Expressing:

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.
    Article Snippet: After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.. The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.. The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.

    TUNEL Assay:

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.
    Article Snippet: After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.. The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.. The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.

    Staining:

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.
    Article Snippet: After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.. The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.. The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.

    Control:

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.
    Article Snippet: After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.After blocking, the membranes were incubated with the primary antibodies overnight at 4◦C in TBST and 3 % BSA.. The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.. The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.The membranes were then washed 3 times with TBST and incubated with secondary antibodies in TBST, 5 % skimmed milk for 1 h at room temperature.



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    goat polyclonal anti chi3l1  (R&D Systems)
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    Goat Polyclonal Anti Chi3l1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    chi3l1  (R&D Systems)
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    Proteomic analysis of ovarian cancer stem cells . A , principal component analysis (PCA) of the proteomic data. B , Volcano plot analysis of the proteomic data. Proteins colored in orange or green are considered up- or down -regulated, respectively. Proteins were ranked by Limma t statistics, and the for the top 30 on each side the gene name is displayed. C , heatmap of log2 fold-changes OCSCs vs bulk for significantly regulated proteins. D , immunoblot analysis for GPNMB and <t>CHI3L1</t> in whole cell lysates from two primary cell lines. Vinculin and GAPDH served as loading controls. E , heatmap of log2 MS intensities for proteins unique to one condition (bulk or OCSCs). Adj.P.Val = Benjamini-Hochberg adjusted p value; PC = principal component.
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    Proteomic analysis of ovarian cancer stem cells . A , principal component analysis (PCA) of the proteomic data. B , Volcano plot analysis of the proteomic data. Proteins colored in orange or green are considered up- or down -regulated, respectively. Proteins were ranked by Limma t statistics, and the for the top 30 on each side the gene name is displayed. C , heatmap of log2 fold-changes OCSCs vs bulk for significantly regulated proteins. D , immunoblot analysis for GPNMB and <t>CHI3L1</t> in whole cell lysates from two primary cell lines. Vinculin and GAPDH served as loading controls. E , heatmap of log2 MS intensities for proteins unique to one condition (bulk or OCSCs). Adj.P.Val = Benjamini-Hochberg adjusted p value; PC = principal component.
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    Immunohistochemical analysis was performed using the IRS scale according to Remmele and Stegner <xref ref-type= 33 to evaluate the expression of the markers. Microvessel density (MVC) was assessed using the Chalkley ocular 34 . The number of macrophages was assessed in the field of view under 200 × magnification. The median value was used as the cut-off value." width="250" height="auto" />
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    Fig. 4. <t>CHI3L1</t> is auto-induced in cells with WT and mutant EGFR. NHBE, A549, H1975 and HCC827 cells were incubated in the presence and absence of rCHI3L1 and the levels of mRNA encoding CHI3L1 and <t>CHI3L1</t> <t>protein</t> accumulation were evaluated. The levels of mRNA encoding CHI3L1 were evaluated using qRT-PCR as illustrated in panel A. The levels of CHI3L1 protein accumulation can be seen in the Western blot evaluations and immunocytochemistry in panel B and C. The values in panel A represent the mean ± SEM of at least triplicate evaluations. The values in panels B and C are representative of at least 3 similar evaluations. (*p < 0.05 by t-test). Scale bar=25μm, it applies to every subpanel of Panel C.
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    chi3l1 rabbit anti human antibody  (Proteintech)
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    Figure 1. High expression of Chitinase-3-like protein 1 <t>(CHI3L1)</t> in pan-cancer and gliomas. (A) Expression of CHI3L1 mRNA in pan-cancer (with significance). (B,E) Expressions of CHI3L1 in glioma and normal brain tissues in the Chinese Glioma Genome Atlas (CGGA) and The Cancer Genome Atlas (TCGA) cohorts, respectively. (C,F) Expressions of CHI3L1 varied with different statuses of isocitrate dehydrogenase (IDH) mutation in CGGA and TCGA cohorts, respectively. (D,G) Overall Survival (OS) of glioma patients with high and low expressions of CHI3L1 in CGGA and TCGA cohorts, respectively. (H,I) Representative immunohistochemistry (IHC) image of CHI3L1 staining in non-tumor brain tissue and glioblastoma (GBM), respectively (ns, non-significant; *, <0.05; **, <0.01; ***, <0.001; ****, <0.0001).
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    Image Search Results


    Proteomic analysis of ovarian cancer stem cells . A , principal component analysis (PCA) of the proteomic data. B , Volcano plot analysis of the proteomic data. Proteins colored in orange or green are considered up- or down -regulated, respectively. Proteins were ranked by Limma t statistics, and the for the top 30 on each side the gene name is displayed. C , heatmap of log2 fold-changes OCSCs vs bulk for significantly regulated proteins. D , immunoblot analysis for GPNMB and CHI3L1 in whole cell lysates from two primary cell lines. Vinculin and GAPDH served as loading controls. E , heatmap of log2 MS intensities for proteins unique to one condition (bulk or OCSCs). Adj.P.Val = Benjamini-Hochberg adjusted p value; PC = principal component.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Quantitative Proteomics and Phosphoproteomics Analysis of Patient-Derived Ovarian Cancer Stem Cells

    doi: 10.1016/j.mcpro.2025.100965

    Figure Lengend Snippet: Proteomic analysis of ovarian cancer stem cells . A , principal component analysis (PCA) of the proteomic data. B , Volcano plot analysis of the proteomic data. Proteins colored in orange or green are considered up- or down -regulated, respectively. Proteins were ranked by Limma t statistics, and the for the top 30 on each side the gene name is displayed. C , heatmap of log2 fold-changes OCSCs vs bulk for significantly regulated proteins. D , immunoblot analysis for GPNMB and CHI3L1 in whole cell lysates from two primary cell lines. Vinculin and GAPDH served as loading controls. E , heatmap of log2 MS intensities for proteins unique to one condition (bulk or OCSCs). Adj.P.Val = Benjamini-Hochberg adjusted p value; PC = principal component.

    Article Snippet: The membranes were incubated overnight at 4 °C with the following primary antibodies: GPNMB (LSBio, cat# LS- B11132 ), CHI3L1 (R&D Systems, cat# AF2599), phospho-PDGFR beta tyrosine-1009 (Cell Signaling Technology, cat# 3124), PDGFR beta (Cell Signaling Technology cat# #4564), GAPDH (Sigma-Aldrich, cat# ABS16), and vinculin (Sigma-Aldrich, cat# V9131).

    Techniques: Western Blot

    Immunohistochemical analysis was performed using the IRS scale according to Remmele and Stegner <xref ref-type= 33 to evaluate the expression of the markers. Microvessel density (MVC) was assessed using the Chalkley ocular 34 . The number of macrophages was assessed in the field of view under 200 × magnification. The median value was used as the cut-off value." width="100%" height="100%">

    Journal: Scientific Reports

    Article Title: New insights into the role of the CHI3L2 protein in invasive ductal breast carcinoma

    doi: 10.1038/s41598-024-77930-5

    Figure Lengend Snippet: Immunohistochemical analysis was performed using the IRS scale according to Remmele and Stegner 33 to evaluate the expression of the markers. Microvessel density (MVC) was assessed using the Chalkley ocular 34 . The number of macrophages was assessed in the field of view under 200 × magnification. The median value was used as the cut-off value.

    Article Snippet: The LSAB + detection system (Dako) was used to visualize IHC reactions obtained with goat polyclonal antibodies against CHI3L1 (1:100, AF2599, 20 min; R&D Systems, Minneapolis, MN USA), VEGFC (1:100, 18 h, 4°C; ReliaTech GmbH, Braunschweig, Germany) and VEGFD (1:100, 18 h, 4°C; ReliaTech GmbH).

    Techniques: Immunohistochemical staining, Expressing, Marker

    Analysis of immunohistochemical expression and correlations of CHI3L2 and A) angiogenic markers: CHI3L1 (Spearman correlation), CD34 and VEGFA (Kendall’s tau-b correlation); B) analysis of expression of CHI3L2, CD68 and CD206 (Spearman correlation); statistically significant p-values were < 0.05.

    Journal: Scientific Reports

    Article Title: New insights into the role of the CHI3L2 protein in invasive ductal breast carcinoma

    doi: 10.1038/s41598-024-77930-5

    Figure Lengend Snippet: Analysis of immunohistochemical expression and correlations of CHI3L2 and A) angiogenic markers: CHI3L1 (Spearman correlation), CD34 and VEGFA (Kendall’s tau-b correlation); B) analysis of expression of CHI3L2, CD68 and CD206 (Spearman correlation); statistically significant p-values were < 0.05.

    Article Snippet: The LSAB + detection system (Dako) was used to visualize IHC reactions obtained with goat polyclonal antibodies against CHI3L1 (1:100, AF2599, 20 min; R&D Systems, Minneapolis, MN USA), VEGFC (1:100, 18 h, 4°C; ReliaTech GmbH, Braunschweig, Germany) and VEGFD (1:100, 18 h, 4°C; ReliaTech GmbH).

    Techniques: Immunohistochemical staining, Expressing

    Fig. 4. CHI3L1 is auto-induced in cells with WT and mutant EGFR. NHBE, A549, H1975 and HCC827 cells were incubated in the presence and absence of rCHI3L1 and the levels of mRNA encoding CHI3L1 and CHI3L1 protein accumulation were evaluated. The levels of mRNA encoding CHI3L1 were evaluated using qRT-PCR as illustrated in panel A. The levels of CHI3L1 protein accumulation can be seen in the Western blot evaluations and immunocytochemistry in panel B and C. The values in panel A represent the mean ± SEM of at least triplicate evaluations. The values in panels B and C are representative of at least 3 similar evaluations. (*p < 0.05 by t-test). Scale bar=25μm, it applies to every subpanel of Panel C.

    Journal: Translational oncology

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.

    doi: 10.1016/j.tranon.2024.102108

    Figure Lengend Snippet: Fig. 4. CHI3L1 is auto-induced in cells with WT and mutant EGFR. NHBE, A549, H1975 and HCC827 cells were incubated in the presence and absence of rCHI3L1 and the levels of mRNA encoding CHI3L1 and CHI3L1 protein accumulation were evaluated. The levels of mRNA encoding CHI3L1 were evaluated using qRT-PCR as illustrated in panel A. The levels of CHI3L1 protein accumulation can be seen in the Western blot evaluations and immunocytochemistry in panel B and C. The values in panel A represent the mean ± SEM of at least triplicate evaluations. The values in panels B and C are representative of at least 3 similar evaluations. (*p < 0.05 by t-test). Scale bar=25μm, it applies to every subpanel of Panel C.

    Article Snippet: The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.

    Techniques: Mutagenesis, Incubation, Quantitative RT-PCR, Western Blot, Immunocytochemistry

    Fig. 6. Effects of CHI3L1 inhibitors and TKI, alone and in combination, on the EGFR axis in vitro. HCC827 cells were incubated with rCHI3L1 and the effects of osimertinib (OSM) and FRG on the expression of CHI3L1, EGF and EGFR were evaluated by qRT-PCR (panels A–C). In panels D and E, H1975 cells were incubated with low doses of osimertinib (OSM) and FRG, alone or in combination, and the levels of mRNA encoding CHI3L1 and EGF were evaluated. In panel F, H1975 cells were incubated with osimertinib (OSM) and FRG antibody, alone or in combination, and the levels of apoptosis were evaluated by TUNEL staining. (*p < 0.05; **p < 0.01; *** p < 0.001, ****p < 0.0001 by ANOVA with multiple comparisons). ns, not significant.

    Journal: Translational oncology

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.

    doi: 10.1016/j.tranon.2024.102108

    Figure Lengend Snippet: Fig. 6. Effects of CHI3L1 inhibitors and TKI, alone and in combination, on the EGFR axis in vitro. HCC827 cells were incubated with rCHI3L1 and the effects of osimertinib (OSM) and FRG on the expression of CHI3L1, EGF and EGFR were evaluated by qRT-PCR (panels A–C). In panels D and E, H1975 cells were incubated with low doses of osimertinib (OSM) and FRG, alone or in combination, and the levels of mRNA encoding CHI3L1 and EGF were evaluated. In panel F, H1975 cells were incubated with osimertinib (OSM) and FRG antibody, alone or in combination, and the levels of apoptosis were evaluated by TUNEL staining. (*p < 0.05; **p < 0.01; *** p < 0.001, ****p < 0.0001 by ANOVA with multiple comparisons). ns, not significant.

    Article Snippet: The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.

    Techniques: In Vitro, Incubation, Expressing, Quantitative RT-PCR, TUNEL Assay, Staining

    Fig. 8. Anti-CHI3L1 and TKI interact to inhibit tumor metastasis. WT mice were challenged with B16-F10 (B16) melanoma cells or control vehicle and treated with control IgG, FRG and or TKIs, alone or in combination. Melanoma lung metastasis was evaluated 2 weeks later. (A) Representative lungs from mice treated with control IgG, FRG and or gefitinib, alone or in combination. (B) The number of pleural melanoma colonies was quantitated in the lungs from the mice in panel A. (C) Representative lungs from mice treated with IgG, FRG and osimertinib, alone and in combination. (D) The number of pleural melanoma colonies was quantitated in the lungs from the mice in panel C. In panels B and D, each dot is representative of an individual animal. Panels A and C are representative of at least 3 evaluations. The values in panels B and D represent the mean ± SEM of the evaluations represented by the individual dots in the lungs from the experiments illustrated in panel A and C respectively. *P < 0.05. ***P < 0.001; ****P < 0.0001. by ANOVA with multiple comparisons). ns, not significant.

    Journal: Translational oncology

    Article Title: Chitinase 3-like-1 (CHI3L1) in the pathogenesis of epidermal growth factor receptor mutant non-small cell lung cancer.

    doi: 10.1016/j.tranon.2024.102108

    Figure Lengend Snippet: Fig. 8. Anti-CHI3L1 and TKI interact to inhibit tumor metastasis. WT mice were challenged with B16-F10 (B16) melanoma cells or control vehicle and treated with control IgG, FRG and or TKIs, alone or in combination. Melanoma lung metastasis was evaluated 2 weeks later. (A) Representative lungs from mice treated with control IgG, FRG and or gefitinib, alone or in combination. (B) The number of pleural melanoma colonies was quantitated in the lungs from the mice in panel A. (C) Representative lungs from mice treated with IgG, FRG and osimertinib, alone and in combination. (D) The number of pleural melanoma colonies was quantitated in the lungs from the mice in panel C. In panels B and D, each dot is representative of an individual animal. Panels A and C are representative of at least 3 evaluations. The values in panels B and D represent the mean ± SEM of the evaluations represented by the individual dots in the lungs from the experiments illustrated in panel A and C respectively. *P < 0.05. ***P < 0.001; ****P < 0.0001. by ANOVA with multiple comparisons). ns, not significant.

    Article Snippet: The primary antibodies used in this study are anti-(a) human CHI3L1 (R&D Systems, MN, US, #AF2599-SP) and anti-KEAP-1(Santa Cruz Biotechnology, TX, US, #8047), β-actin (Santa Cruz Biotechnology, TX, US, # Sc47778) antibodies.

    Techniques: Control

    Figure 1. High expression of Chitinase-3-like protein 1 (CHI3L1) in pan-cancer and gliomas. (A) Expression of CHI3L1 mRNA in pan-cancer (with significance). (B,E) Expressions of CHI3L1 in glioma and normal brain tissues in the Chinese Glioma Genome Atlas (CGGA) and The Cancer Genome Atlas (TCGA) cohorts, respectively. (C,F) Expressions of CHI3L1 varied with different statuses of isocitrate dehydrogenase (IDH) mutation in CGGA and TCGA cohorts, respectively. (D,G) Overall Survival (OS) of glioma patients with high and low expressions of CHI3L1 in CGGA and TCGA cohorts, respectively. (H,I) Representative immunohistochemistry (IHC) image of CHI3L1 staining in non-tumor brain tissue and glioblastoma (GBM), respectively (ns, non-significant; *, <0.05; **, <0.01; ***, <0.001; ****, <0.0001).

    Journal: International journal of molecular sciences

    Article Title: CHI3L1 as a Prognostic Biomarker and Therapeutic Target in Glioma.

    doi: 10.3390/ijms25137094

    Figure Lengend Snippet: Figure 1. High expression of Chitinase-3-like protein 1 (CHI3L1) in pan-cancer and gliomas. (A) Expression of CHI3L1 mRNA in pan-cancer (with significance). (B,E) Expressions of CHI3L1 in glioma and normal brain tissues in the Chinese Glioma Genome Atlas (CGGA) and The Cancer Genome Atlas (TCGA) cohorts, respectively. (C,F) Expressions of CHI3L1 varied with different statuses of isocitrate dehydrogenase (IDH) mutation in CGGA and TCGA cohorts, respectively. (D,G) Overall Survival (OS) of glioma patients with high and low expressions of CHI3L1 in CGGA and TCGA cohorts, respectively. (H,I) Representative immunohistochemistry (IHC) image of CHI3L1 staining in non-tumor brain tissue and glioblastoma (GBM), respectively (ns, non-significant; *, <0.05; **, <0.01; ***, <0.001; ****, <0.0001).

    Article Snippet: The CHI3L1 rabbit anti-human antibody (12036-1-AP) was purchased from Proteintech (Wuhan, China).

    Techniques: Expressing, Mutagenesis, Immunohistochemistry, Staining

    Figure 2. Differentially expressed genes (DEGs) in gliomas based on CHI3L1 expression. (A) Volcano plot of DEGs. (B) Enrichment of DEGs based on Gene Ontology (GO). (C) Gene set enrichment analysis (GSEA) of DEGs based on Kyoto Encyclopedia of Genes and Genomes (KEGG). (D) Heatmap of 51genes selected from GO enchainment analysis. (E) Univariate Cox regression analysis of 51 genes (with statistical significance).

    Journal: International journal of molecular sciences

    Article Title: CHI3L1 as a Prognostic Biomarker and Therapeutic Target in Glioma.

    doi: 10.3390/ijms25137094

    Figure Lengend Snippet: Figure 2. Differentially expressed genes (DEGs) in gliomas based on CHI3L1 expression. (A) Volcano plot of DEGs. (B) Enrichment of DEGs based on Gene Ontology (GO). (C) Gene set enrichment analysis (GSEA) of DEGs based on Kyoto Encyclopedia of Genes and Genomes (KEGG). (D) Heatmap of 51genes selected from GO enchainment analysis. (E) Univariate Cox regression analysis of 51 genes (with statistical significance).

    Article Snippet: The CHI3L1 rabbit anti-human antibody (12036-1-AP) was purchased from Proteintech (Wuhan, China).

    Techniques: Expressing

    Figure 4. Prognostic model construction. (A) Multivariate Cox regression analysis for ORGI risk score, CHI3L1, and clinical status (grade, age, IDH mutation status, co-deletion status of 1p/19q chromosomes) (CGGA cohort). (B–D) Receiver operating characteristic (ROC) curves of ORGI risk and clinical factors for 1-, 3-, and 5-year OS prediction (CGGA cohort). (E) Prognostic model of ORGI risk score, expression of CHI3L1, and clinical factors in nomogram (CGGA cohort). (F,G) Calibration curves showing the concordance between predicted and observed 1-, 3-, and 5-year OS in CGGA and TCGA cohorts.

    Journal: International journal of molecular sciences

    Article Title: CHI3L1 as a Prognostic Biomarker and Therapeutic Target in Glioma.

    doi: 10.3390/ijms25137094

    Figure Lengend Snippet: Figure 4. Prognostic model construction. (A) Multivariate Cox regression analysis for ORGI risk score, CHI3L1, and clinical status (grade, age, IDH mutation status, co-deletion status of 1p/19q chromosomes) (CGGA cohort). (B–D) Receiver operating characteristic (ROC) curves of ORGI risk and clinical factors for 1-, 3-, and 5-year OS prediction (CGGA cohort). (E) Prognostic model of ORGI risk score, expression of CHI3L1, and clinical factors in nomogram (CGGA cohort). (F,G) Calibration curves showing the concordance between predicted and observed 1-, 3-, and 5-year OS in CGGA and TCGA cohorts.

    Article Snippet: The CHI3L1 rabbit anti-human antibody (12036-1-AP) was purchased from Proteintech (Wuhan, China).

    Techniques: Mutagenesis, Expressing

    Figure 5. Function verification of CHI3L1 in vitro. (A) Verification of silencing efficacy of CHI3L1 shRNA using qPCR. (B,C) Protein quantity results of CHI3L1 knockdown in U87MG. (D,E) Colony formation assay for evaluation of proliferation between U87MG and U87MG_sh1. (F–P) qPCR assay for identification of ORGI mRNA regulation after CHI3L1 knockdown (NOX4, SH3PXD2B, SYK, PRDX1, GNAI3, LOXL3, MAOB, CYBB, HVCN1, ASPHD, GLUD1) (ns, non-significant; *, <0.05; **, <0.01; ***, <0.001; ****, <0.0001).

    Journal: International journal of molecular sciences

    Article Title: CHI3L1 as a Prognostic Biomarker and Therapeutic Target in Glioma.

    doi: 10.3390/ijms25137094

    Figure Lengend Snippet: Figure 5. Function verification of CHI3L1 in vitro. (A) Verification of silencing efficacy of CHI3L1 shRNA using qPCR. (B,C) Protein quantity results of CHI3L1 knockdown in U87MG. (D,E) Colony formation assay for evaluation of proliferation between U87MG and U87MG_sh1. (F–P) qPCR assay for identification of ORGI mRNA regulation after CHI3L1 knockdown (NOX4, SH3PXD2B, SYK, PRDX1, GNAI3, LOXL3, MAOB, CYBB, HVCN1, ASPHD, GLUD1) (ns, non-significant; *, <0.05; **, <0.01; ***, <0.001; ****, <0.0001).

    Article Snippet: The CHI3L1 rabbit anti-human antibody (12036-1-AP) was purchased from Proteintech (Wuhan, China).

    Techniques: In Vitro, shRNA, Knockdown, Colony Assay